Induction Of Callus Formation In Gapi Banana (Musa Acuminata L.) Ovaries At Various Concentrations Of Iaa And Bap In Vitro
Keywords:
Plant tissue culture, PGR, Browning, MS Medium
Abstract
Gapi banana (Musa acuminata L.) is a valuable tropical fruit limited by a shortage of quality planting material. In vitro callus induction from floral ovary explants was examined in this work to determine the effects of coupled IAA and BAP. Sterilized explants were cultured on MS media across five IAA+BAP combinations: A0 (0/0 ppm), A1 (2/8 ppm), A2 (5/8 ppm), A3 (8/8 ppm), and A4 (11/8 ppm). Using a completely randomized design, data on explant viability, callus formation, and browning were evaluated via ANOVA and Chi-square tests. While treatment combinations did not significantly alter explant survival, theysignificantly affected callus formation and browning. Treatments featuring 8–11 ppm IAA alongside 8 ppm BAP consistently maximized callus induction through 7 weeks post-inoculation, highlighting this hormone combination as an effective strategy for Gapi banana micropropagation.
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Zhang, Q. (2024). On the asymptotic distributions of some test statistics for two-way contingency tables. arXiv Preprint. https://doi.org/ 10.48550/arXiv.2409.14255
Darvari, F. M., Sariah, M., Puad, M. P., & Maziah, M. (2010). Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers. African Journal of Biotechnology, 9(16), 2360–2366. Djarot, P., & Gati, L. E. (2024). Aplikasi Teknik Kultur In Vitro (B. Trim, Ed.; 1st ed.). CV Penulis Profesional Indonesia.
Djarot, P., & Gati, L. E. (2024). Aplikasi Teknik Kultur In Vitro (B. Trim, Ed.; 1st ed.). CV Penulis Profesional Indonesia. https://www.researchgate.net/publication/385974032
Dwivanny, F., Wikantika, K., Susanto, A., Ghazali, F., Lim, C., & Kamalesha, G. (2021). PISANG INDONESIA. ITB Press.
Dwiyani, R., & Yuswati, H. (2018). Callus induction from banana flower explants using 2,4-Dichlorophenoxyacetic acid (2,4-D). Jurnal Biologi Udayana, 22(2), 70–74.
Elma, T. A., Suminar, E., Mubarok, S., & Nuraini, A. (2017). Micro-shoot multiplication of 'Raja Bulu' banana (Musa paradisiaca L.) in vitro under various types and concentrations of cytokinins. Jurnal Kultivasi, 16(3), 418–424.
Faisal, M., Ahmad, N., Anis, M., Alatar, A. A., & Qahtan, A. A. (2018). Auxin-cytokinin synergism in vitro for producing genetically stable plants of Ruta graveolens using shoot tip meristems. Saudi Journal of Biological Sciences, 25(2), 273–277. https://doi.org/10.1016/j.sjbs. 2017.09.009
Febriani, D. (2022). Browning Rate in Callus Induction from Banana Leaf Explants (Musa acuminata C. var. Mas Kirana) In Vitro [Undergraduate thesis, Universitas Tidar].
George, E. F., Hall, M. A., & Klerk, G.-J. de. (2008). Plant Propagation by Tissue Culture (3rd ed.). Springer. https://doi.org/ 10.1007/978-1-4020-5005-3 Hutami, S. (2008). Browning problems in tissue culture. Jurnal AgroBiogen, 4(2), 83–88.
Isah, T. (2015). Adjustments to in vitro culture conditions and associated anomalies in plants. Acta Biologica Cracoviensia Series Botanica, 57(2), 9–28. https://doi.org/10.1515/abcsb-2015-0026
Hutami, S. (2008). Masalah Pencoklatan pada Kultur Jaringan. AGROBIOGEN, 4(2), 83–88. http://ejurnal.litbang.pertanian.go.id/index.php/ja
Khasanah, N. K., Ahmad, D., & Hasyim, A. D. (2024). Horticultural Statistics of Gorontalo Province (Vol. 8). CV. Rivaldi.
Lestari, E. G. (2011). The role of plant growth regulators in plant propagation through tissue culture. Jurnal AgroBiogen, 7(1), 63–68. Liu, C., Fan, H., Zhang, J., Wu, J., Zhou, M., Cao, F., Tao, G., & Zhou, X. (2024). Combating browning: mechanisms and management strategies in in vitro culture of economic woody plants. Forestry Research, 4, Article e0024. https://doi.org/10.48130/forres-0024-0026
Liu, C., Fan, H., Zhang, J., Wu, J., Zhou, M., Cao, F., Tao, G., & Zhou, X. (2024). Combating browning: mechanisms and management strategies in in vitro culture of economic woody plants. In Forestry Research (Vol. 4). Maximum Academic Press.
Rohman, F., Sukri, M. Z., Firgiyanto, R., Rohman, H. F., & Robbi, M. N. F. (2024). Effect of IAA and BAP on the growth of Cavendish banana (Musa acuminata L.) explants via in vitro culture. Vegetalika, 13(3), 281–292. https://doi.org/10.22146/veg.83613
Sadat, M. S., Siregar, L. A. M., & Setiado, H. (2018). Effect of IAA and BAP on micro-shoot induction from corm explants of Kepok banana (Musa paradisiaca L.). Jurnal Agroekoteknologi FP USU, 6(1), 107–112.
Sari, R. P., Suwirmen, & Idris, M. (2013). Micro-shoot multiplication of Tetrastigma rafflesiae Miq. on Murashige-Skoog medium with additions of BAP and NAA in vitro. Jurnal Biologi Universitas Andalas, 2(4), 258–261.
Thiry, M., Iken, M., Renaut, J., Lutts, S., & Guerriero, G. (2026). Impact of plant growth regulators on callus induction in Cannabis sativa L. Cells, 15(4), Article 385. https://doi.org/10.3390/cells15040385
Wahidah, F. B., & Hasrul. (2017). Effect of Indole Acetic Acid (IAA) plant growth regulator on the in vitro growth of Sayang banana (Musa paradisiaca L. var. Sayang). Teknosains, 11(1), 27–41.
Zhang, Q. (2024). On the asymptotic distributions of some test statistics for two-way contingency tables. arXiv Preprint. https://doi.org/ 10.48550/arXiv.2409.14255
Published
2026-08-02
How to Cite
A. Anggu, M., Husain, I., Dama, H., Zakaria, F., & Apriliani, S. (2026). Induction Of Callus Formation In Gapi Banana (Musa Acuminata L.) Ovaries At Various Concentrations Of Iaa And Bap In Vitro. International Journal of Technology and Education Research, 4(03), 775-784. https://doi.org/10.63922/ijeter.v4i03.5524
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