Effectiveness of Hydrogel of Green Grasshopper Leaf Extract (Cyclea barbata) and Breadfruit Leaf (Artocarpus altilis) against Burns in Rabbits (Oryctolagus cuniculus)
Keywords:
green grasshopper leaf extract, breadfruit leaf extract, hydrogel, burns
Abstract
This study aimed to evaluate the effectiveness of a hydrogel formulation combining green grasshopper leaf extract (Cyclea barbata) and breadfruit leaf extract (Artocarpus altilis) in accelerating burn wound healing in rabbits (Oryctolagus cuniculus). The study used a completely randomized design with five treatments and five replications. Burn wounds were created on the rabbits’ dorsal area and treated with various hydrogel formulations, including a negative control and a positive control using Bioplacenton. The observed parameters included changes in wound diameter, clinical signs of inflammation such as redness, swelling, and exudate, and the duration of healing. The results showed that the combined hydrogel of green grasshopper leaf extract and breadfruit leaf extract accelerated burn wound healing compared with the negative control, with the best result observed in treatment P1, which achieved complete healing on day 11 and produced a response close to that of the positive control. No local adverse effects such as excessive erythema, edema, or purulent exudate were observed in any treatment group. These findings indicate that the hydrogel combination of Cyclea barbata and Artocarpus altilis extracts has potential as a safe, effective, and economical veterinary herbal preparation for burn treatment in rabbits and other livestock.
References
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Patenall, B. L., Carter, K. A., & Ramsey, M. R. (2024). Kick-Starting Wound Healing : A Review of Pro-Healing Drugs.
Puruhita, R., Aldila, S., & Ariyani, D. P. (2025). INDONESIAN JOURNAL OF PHARMACY & SCIENCE Clove Leaf Ethanol ( Syzygium aromaticum L .) In rabbits ( Oryctolagus cuniculus ). 8(1), 221–228. https://doi.org/10.52216/jfsi.vol8no1p221-228
Sari, P. R., Kurniawan, Y., & Layal, K. (2021). Formulation of Breadfruit Leaf Extract Gel Preparation (Artocarpus altilis) as Burn Healing in Rabbits with Variation in Carbopol 940 Concentration as a Gelling Agent. As-Shifaa: Journal of Pharmacy, 13(2), 130–140. https://doi.org/10.56711/jifa.v13i2.781
Sembiring, N., Nasution, A. N., Syahrian, M. F., Mukhtar, Z., Putri, L. D., Tarigan, A. I., & Girsang, E. (2023). Test of Breadfruit Leaf Extract Cream Preparation ( Artocarpus Altilis ) and Antioxidant Activity Test with DPPH Method. 3(7), 1601–1619.
Smith, J., & Rai, V. (2024). Differentiation of Fibroblasts , Keratinocytes , and Vascular Smooth Muscle Cells during Wound Healing.
Syahadatun, I., Hidayat, R., Septiarini, A. D., Duta, U., Surakarta, B., Health, F. I., Duta, U., Surakarta, B., Info, A., Extract, B. L., Wound, C., & Healing, W. (2025). TEST THE EFFECTIVENESS OF ETHANOL EXTRACT 70% OF BREADFRUIT LEAF (Artocarpus altilis) ON THE HEALING OF CUT WOUNDS IN MALE WHITE RABBITS. 9(1), 25–33.
Zulkefli, N., Nur, C., Che, M., Sayuti, N. H., Kamarudin, A. A., Saad, N., Hamezah, H. S., Bunawan, H., & Baharum, S. N. (2023). Flavonoids as Potential Wound-Healing Molecules : Emphasis on Pathways Perspective.
Akmalia, I. D., Prayogo, M. S., & Wulandari, S. (2025). Practicum on the Utilization and Processing of Green Grasshopper Leaves ( Cyclea Barbata ) IPAS Subject Class V at Mi Jami ' yatul Mubtadi ' in. 2.
Astryna, S. Y., & Samaniyah, S. (2025). LUKA BAKAR DERAJAT II Anti-Inflammatory Activity Of Breadfruit Leaf Ethanol Extract Nanogel On Male White Rats Suffering From Second Degree Burns. 11(1), 622–627.
Asyifa, T. N., Mustofa, S., Ismunandar, H., Utama, W. T., Biochemistry, B., Medicine, F., & Lampung, U. (2023). Ways To Speed Up Wound Healing How To Promote Wound Healing. 12, 659–666.
Badriyah, L. (2023). UTOMO HEALTH SCIENCE ESSAY. XVII(2), 46–51.
Brahmantiyo, B., Pratiwi, N., Saputra, F., Raharjo, Y. C., & Prasetyo, L. H. (2021). Growth Performance of Male Line of Superior Tropical Rabbit. September.
Cioce, A., Cavani, A., Cattani, C., & Scopelliti, F. (2024). Role of the Skin Immune System in Wound Healing.
Dinda. (2023). Antibacterial Ethanol Extract of Green Grasshopper Leaves ( Cyclea barbata Miers ) Against Extended Spectrum Beta Lactamase Producing Escherichia coli (ESBL). 4(1), 22–27.
Djamil, M., Arja, A., Mustofa, S., Medicine, F., Lampung, U., Biochemistry, B., Medicine, F., & Lampung, U. (2026). Burns: Epidemiology, Pathophysiology, and Factors That Accelerate Healing. 16(April), 106–113.
Erega. (2005). Systematics and Species Limits of Breadfruit ( Artocarpus , Moraceae ). 30, 603–615.
Febriani, A., Maruya, I., & Sulistyaningsih, F. (2020). Formulation and Irritation Test of Gel Preparation Combination of Kencur Rhizome Ethanol Extract ( Kaempferia galanga L .) and Gotu Herbal Ethanol Extract ( Centella asiatica ( L .) Urban ). 13(1).
Febrianto, S., Praharsini, F. V., Annas, Z. F., & Isneni, N. (2022). Cyclea barbata L. Miers.: Traditional uses, phytochemicals, and. 3(2).
Firdaus, M. N. A., Kardela, W., & Ifora, I. (2022). Phytochemical and Anti-Inflammatory Potential of Anredera cordifolia (Ten): A Review. Journal of Drug Delivery and Therapeutics, 12(2), 121–125. https://doi.org/10.22270/jddt.v12i2.5228
Fitrya, F., Muharni, Wahyuni, F. S., & Amriani, A. (2023). Cytotoxicity and anti-inflammatory activity of ethanol extract of Artocarpus altilis (Parkinson ex F.A.Zorn) Fosberg leaf in lipopolysaccharide-stimulated RAW 264.7 cells. Journal of Pharmacy & Pharmacognomy Research, 11(3), 517–522. https://doi.org/10.56499/jppres23.1623_11.3.517
Hakim, I. R., Lestari, F., & Priani, S. E. (2010). Literature Review of Plants with Potential in Healing Burns. 14–20.
Hilaria, M., & Uhe, A. L. (2014). The effect of propolis extract concentration on the healing effect of burns on the back skin of rabbits.
Jittiporn, K., Samer, J., & Thitiwuthikiat, P. (2024). α -Mangostin protects endothelial cell against H 2 O 2 -induced senescence via p38 / Sirt1 / MnSOD pathway. 14(05), 91–97. https://doi.org/10.7324/JAPS.2024.161374
Kurniawan, Y., Layal, K., Medicine, F., & Muhammadiyah, U. (2017). Giving Breadfruit Leaf Extract Gel (Artocarpus altilis) Can Accelerate the Burn Healing Process in Mice Introduction. 8(1).
Lebas, F., Coudert, P., Rouvier, R., & de Rochambeau, H. (1986). The Rabbit: Husbandry, Health, and Production. FAO Animal Production and Health Series No. 21. Rome: Food and Agriculture Organization of the United Nations. 202 pp. ISBN 92-5-101253-9.
Li, M., Xia, W., Khoong, Y. M., Huang, L., Huang, X., Liang, H., Zhao, Y., & Mao, J. (2023). Smart and versatile biomaterials for cutaneous wound healing. Biomaterials Research, 1–32. https://doi.org/10.1186/s40824-023-00426-2
Maulida, J., Sihotang, S. H., & Nadia, S. (2024). SCREENING AND TESTING OF ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACT OF BREADFRUIT LEAVES ( Artocarpus altilis ) BY FRAP ( FERRIC REDUCING ANTIOXIDANT POWER ) METHOD forte Journal , Vol . 04 , No . 01 , January 2024. 04, 13–19.
Nasihin, A. (2025). Bulletin of Tropical Livestock Vegetables as Rabbit Feed: between Benefits and Health Risks. 6(1), 1–9.
Nurhidayat. (2023). Effect of the Use of Auxin Growth Regulator (IBA) on the Growth of Green Grasshopper Stem Cuttings (Cyclea Barbata Miers). 6(2), 1–6.
Patenall, B. L., Carter, K. A., & Ramsey, M. R. (2024). Kick-Starting Wound Healing : A Review of Pro-Healing Drugs.
Puruhita, R., Aldila, S., & Ariyani, D. P. (2025). INDONESIAN JOURNAL OF PHARMACY & SCIENCE Clove Leaf Ethanol ( Syzygium aromaticum L .) In rabbits ( Oryctolagus cuniculus ). 8(1), 221–228. https://doi.org/10.52216/jfsi.vol8no1p221-228
Sari, P. R., Kurniawan, Y., & Layal, K. (2021). Formulation of Breadfruit Leaf Extract Gel Preparation (Artocarpus altilis) as Burn Healing in Rabbits with Variation in Carbopol 940 Concentration as a Gelling Agent. As-Shifaa: Journal of Pharmacy, 13(2), 130–140. https://doi.org/10.56711/jifa.v13i2.781
Sembiring, N., Nasution, A. N., Syahrian, M. F., Mukhtar, Z., Putri, L. D., Tarigan, A. I., & Girsang, E. (2023). Test of Breadfruit Leaf Extract Cream Preparation ( Artocarpus Altilis ) and Antioxidant Activity Test with DPPH Method. 3(7), 1601–1619.
Smith, J., & Rai, V. (2024). Differentiation of Fibroblasts , Keratinocytes , and Vascular Smooth Muscle Cells during Wound Healing.
Syahadatun, I., Hidayat, R., Septiarini, A. D., Duta, U., Surakarta, B., Health, F. I., Duta, U., Surakarta, B., Info, A., Extract, B. L., Wound, C., & Healing, W. (2025). TEST THE EFFECTIVENESS OF ETHANOL EXTRACT 70% OF BREADFRUIT LEAF (Artocarpus altilis) ON THE HEALING OF CUT WOUNDS IN MALE WHITE RABBITS. 9(1), 25–33.
Zulkefli, N., Nur, C., Che, M., Sayuti, N. H., Kamarudin, A. A., Saad, N., Hamezah, H. S., Bunawan, H., & Baharum, S. N. (2023). Flavonoids as Potential Wound-Healing Molecules : Emphasis on Pathways Perspective.
Published
2026-06-30
How to Cite
Fiqqih Shahdan, I Putu Juli Sukariada, & Yohana Kamlasi. (2026). Effectiveness of Hydrogel of Green Grasshopper Leaf Extract (Cyclea barbata) and Breadfruit Leaf (Artocarpus altilis) against Burns in Rabbits (Oryctolagus cuniculus) . International Journal of Education, Vocational and Social Science , 5(03), 3012-3022. https://doi.org/10.63922/ijevss.v5i03.4474
Contact Person +6282139474255
Email : ijevss@citakonsultindo.or.id / editor.ijevss@gmail.com
